1. Bishop Thomas De Castro
- Apostolic Vicar of Kanara 1674-1684.
- Ordained Bishop on Spetember 15, 1675.
- Appointed Apostolic Vicar on August 30,1675.
- Died at Mangalore on July 16, 1684 and buried at Milagres.
2. Bishop Bernardine of St Agnes, O.C.D.
- Pro-Vicar Apostolic of Mangalore 1845-1853.
- Appointed on May 5, 1845.
- Died at Rome, on March 15, 1853.
3. Bishop Michael Antony of St Louis O.C.D.
- Vicar Apostolic of Mangalore 1853-1870.
- Appointed on March 15, 1853.
- Resigned the See in 1870.
- Died in Quilon 1878.
4. Bishop Mary Ephrem of the Sacred Heart of Jesus, O.C.D.
- Vicar Apostolic of Mangalore 1870-1873.
- Appointed on June 3, 1870.
- Died in Mangalore on April 10, 1873
- Buried at the Cathedral.
5. Bishop Nicholas Maria Pagani S.J.
- Vicar Apostolic 1878-1886.
- He was consecrated Bishop, on October 25, 1885.
- On proclamation of Hierarchy for India, he became the First Bishop of Mangalore on September 1, 1886.
- He died in Mangalore on April 30, 1895.
- Buried at the Cathedral.
6. Bishop Abundius Cavadini S.J.
- Bishop of Mangalore 1895-1910.
- Ordained Bishop on November 26, 1895.
- Died at Ootacamund on March 26, 1910
- Buried at the Cathedral.
7. Bishop Paul Perini S.J.
- Bishop of Mangalore 1910-1923.
- Appointed on August 17, 1910.
- Ordained Bishop on December 4, 1910.
- He was transferred to the newly constituted Bishopric of Calicut, but continued as Administrator Apostolic of Mangalore 1923-1928.
- Died at Bangalore on June 28, 1932.
- Buried at the Calicut Cathedral on June 30, 1932.
8. Bishop Valerian J. D’Souza
- Bishop of Mangalore 1928-1930.
- Ordained Bishop on April 15, 1928.
- Died at Waterford, Ireland on August 14, 1930.
- Buried at the Cathedral on September 29, 1930.
9. Bishop Victor R. Fernandes
- Bishop of Mangalore 1931-1955.
- Appointed on May 6, 1931.
- Ordained Bishop on September 21, 1931.
- Died on January 4, 1955.
- Buried at the Cathedral.
10. Bishop Basil S. T. Peres
- Coadjutor Bishop to Bishop Victor Fernandes.
- Ordained Bishop on October 28, 1952.
- As Bishop of Mangalore from January 4, 1955 till his death on April 28, 1958
- Buried at Rosario Cathedral.
11. Bishop Raymond D’Mello
- Bishop of Mangalore from April 6, 1959 till 1964.
- Transferred to Allahabad on April 21, 1964;
- Died on November 24, 1971.
- Buried in Allahabad Cathedral.
12. Bishop Basil S. D’Souza
- Bishop of Mangalore 1965-1996.
- Appointed Bishop on March 22, 1965.
- Ordained on May 11, 1965.
- Died on September 5, 1996.
- Buried on September 9, 1996 at Rosario Cathedral.
13. Bishop Aloysius Paul D’Souza
- Bishop of Mangalore 1996-2018.
- Ordained Bishop on May 15, 1996
- He served as Auxiliary Bishop to Bishop Basil D'Souza, after whose death he was elected as Diocesan Administrator on September 13, 1996.
- Nominated Bishop of the Diocese on December 18, 1996
- Installed as Bishop of Mangalore on December 27, 1996.
- His resignation from office became effective on July 3, 2018 when the Holy See appointed Msgr. Peter Paul Saldanha as Bishop of Mangalore.
14. Bishop Peter Paul Saldanha
- Ordained and installed as Bishop of Mangalore on September 15, 2018 by Most Rev. Dr. Aloysius Paul D’Souza at the Rosario Cathedral.
- He served as Asst. Parish Priest in Belle, Milagres-Mangalore and Vittal.
- He was appointed on the staff of St Joseph’s Seminary.
- He pursued higher studies in Rome, and then served as the Professor in Urbaniana University until his appointment as Bishop on July 3, 2018.